Borrelia (Elispot 2)

Description

Borrelia (Elispot 2)
ELISPOT (Enzyme-linked immunosorbent spot)-a method for detecting cells (T-lymphocytes) secreting cytokines.

The method is highly sensitive and can detect even one single T-cell reacting to the infection.

The second-generation EliSpot test is used in the diagnosis of Lyme disease (borreliosis). It assesses the cellular response (antigen-specific T cells) in Lyme disease. The test uses six Borrelia antigens (including 5 recombinant antigens).

EliSpot assays are recommended approximately 6 weeks after treatment completion to assess the effectiveness of the treatment.

Antigens, Borrelia Elispot test system 2:

- The native antigen of Borrelia burgdorferi sensu stricto lysate (strain B31),
- VlsE antigen mixture (mixture of recombinant VlsE proteins from 3 spirochete species: B. burgdorferi ss, B. afzelii and B. garinii), p41i antigen (internal fragment specific for Borrelia burgdorferi sl, as opposed to the complete p41 protein molecule),
- LFA-1 peptide.
- OspA antigen (recombinant),
- OspC mixture (a mixture of recombinant OspC proteins from 3 spirochete species: B. burgdorferi ss, B. afzelii, and B. garinii),